Indirect immunofluorescence of fertilin and cyritestin on sperm surface. Live sperm were stained with antisera m-AS1 or mCyri-AS1 at dilution 1:50 followed by the Fab fragment of rhodamine-conjugated goat anti-mouse IgG. The images are representative of the major pattern observed in each sperm population. Micrographs are a combined transmission image (green) and rhodamine-stained image (red). Fertilin: (A) acrosome intact sperm; (C) acrosome reacted sperm. Cyritestin: (B) acrosome intact sperm; (D) acrosome reacted sperm.

Figure taken from: Yuan, R., Primakoff, P. and Myles, D.G. 1997. A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusion. J. Cell Biol. 137: 105-12.