Powers Lab UC Davis
1. Dilute Bradford Assay solution with dI H2O 1:4 (in a 15 ml or 50 ml conical
tube, for example, and then store @ RT).
2. Put 1 ml of this diluted solution into 1.5 ml microfuge tubes. Prepare tubes in triplicate for each sample to be quantitated.
3. Add 0.5, 1, and 2 ul of sample to each of the three microfuge tubes.
4. Invert to mix.
5. Take spectrophotometric reading at O.D. 595
6. If within the range of 0.1 - 0.8, then do the following calculation:
For 1 ul sample:
O.D. 595 x 40 = _____ mg/ml
For 0.5 ul sample:
O.D. 595 x 40 x 2 = _____ mg/ml
For 2 ul sample:
O.D.595 x 40/2 = ______ mg/ml
If not within the range of 0.1 - 0.8, then adjust amount of sample used and
take another reading.