Kellogg Yeast Extract Buffer (for complexes) with physiological salt

From Powers Lab UC Davis

Reagent

100 mL

10 mL
H2O 67.75 mL 6.78 mL
50 mM Hepes-KOH, 7.6 5 mL 1M stock 0.5 mL
100 mM B-glycero-PO4 10 mL 1M stock 1 mL
50 mM NaF 6.7 mL 0.75 M stock 0.67 mL
1 mM MgCl2 100 uL 1M stock 10 uL
1 mM EGTA 200 uL 0.5M stock 20 uL
5% glycerol 5 mL stock 0.5 mL
0.25% Tween-20 250 uL stock 25 uL
150 mM KCl 5 mL 3M stock 0.5 mL

 

Into the 10mL Buffer add 20ul 1M DTT, 10ul leupeptin, 10ul aprotinin, 100ul PMSF