From Powers Lab UC Davis
1. Put patch of cells from plate into 1.5 ml microfuge tube containing 100µl LiOAc mix.
2. Boil 10µl of salmon sperm DNA 5 minutes.
3. Add salmon sperm DNA and plasmid DNA (about 10 µl of Qiagen miniprep) to cells.
4. Add 700µl of PEG solution to cells and mix; incubate at 30°C for 1 hr.
5. Heat shock 5 minutes at 42°C.
6. Spin down cells (8000 rpm, 5 min).
7. Resuspend in 100µl H2O and plate on selective plates.
LiOAc Mix
1 ml 10X TE
1 ml 1M LiOAc
8ml sterile H2O
PEG Mix
8 ml 50% PEG 3000
1 ml 10X TE
1 ml 1M LiOAc