SELECTED PUBLICATIONS

 

  

 

Scholey, J.M., B. Neighbors, J.R. McIntosh and E.D. Salmon (1984)  Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs. Journal of Biological Chemistry 259:6516-6525. PDF

 

Scholey, J.M., M.E. Porter, P.M. Grissom and J.R. McIntosh (1985)  Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.  Nature 318:483-486.

 

Porter, M.E., J.M. Scholey, D.L. Stemple, G.P.A. Vigers, R.D. Vale, M.P. Sheetz and J.R. McIntosh (1987) Characterization of the microtubule movement produced by sea urchin egg kinesin.  Journal of Biological Chemistry 262:2794-2802.  PDF

 

Leslie, R.J., R.B. Hird, L. Wilson, J.R. McIntosh and J.M. Scholey (1987)  Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.  Proceedings of the National Academy of Sciences (USA) 84:2771-2775.  PDF

 

Lye, R.J., M.E. Porter, J.M. Scholey and J.R. McIntosh (1987)  Identification of a microtubule-based cytoplasmic motor in the nematode C. elegansCell 51:309-318.  PDF

 

Cohn, S.A., A.L. Ingold and J.M. Scholey (1987)  Correlation between the ATPase and microtubule translocating activities of sea urchin egg kinesin.  Nature 328:160-163. PDF

 

Saxton, W.M., M.E. Porter, S.A. Cohn, J.M. Scholey, E.C. Raff and J.R. McIntosh (1988)  Drosophila kinesin:  Characterization of microtubule motility and ATPase.  Proceedings of the National Academy of Sciences 85:1109-1113.  PDF

 

Ingold, A.L., S.A. Cohn and J.M. Scholey (1988)  Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.  Journal of Cell Biology 107:2657-2667.  PDF

 

Cohn, S.A., A.L. Ingold and J.M. Scholey (1989)  Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.  Journal of Biological Chemistry 264:4290-4297.  PDF

 

Scholey, J.M., J. Heuser, J.T. Yang and L.S.B. Goldstein (1989)  Identification of globular mechanochemical heads of kinesin.  Nature 338:355-357. PDF

 

Wright, B.D., J.H. Henson, K.P. Wedaman, P.J. Willy, J.N. Morand and J.M. Scholey (1991)  Subcellular localization and sequence of a sea urchin kinesin heavy chain:  Evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.  Journal of Cell Biology 113:817-833.  PDF

 

Cole, D.G., W.Z. Cande, R.J. Baskin, D.A. Skoufias, C.J. Hogan and J.M. Scholey (1992)  Isolation of a sea urchin egg kinesin-related protein using peptide antibodies.  Journal of Cell Science 101:291-301. PDF

 

Wedaman, K.P., A.E. Knight, J. Kendrick-Jones and J.M. Scholey (1993) Sequences of sea urchin kinesin light chain isoforms.  Journal of Molecular Biology 231:155-158. PDF

 

Hogan, C.J., H. Wein, L. Wordeman, J.M. Scholey, K.E. Sawin and W.Z. Cande (1993)  Inhibition of anaphase spindle elongation "in vitro" by a peptide antibody that recognizes kinesin motor domain. Proceedings of the National Academy of Sciences (USA) 90:6611-6615.  PDF

 

Wright, B.D., M. Terasaki and J.M. Scholey (1993)  Roles of kinesin and kinesin-  like proteins in sea urchin early embryonic cell divisions: Evaluation using antibody microinjection.  Journal of Cell Biology 123:681-689.  PDF

 

Cole, D.G., D.S. Chinn, K.P. Wedaman, K. Hall, T. Vuong and J.M. Scholey (1993)  Novel heterotrimeric kinesin related protein purified from sea urchin eggs. Nature 366:268-270. PDF

 

Skoufias, D.A., D.G. Cole, K. P. Wedaman and J.M. Scholey (1994)  The carboxyl-terminal domain  of kinesin heavy chain is important for membrane binding.  Journal of  Biological Chemistry 269:1477-1485. PDF

 

Cole, D.G., W.M. Saxton, K.B. Sheehan and J.M. Scholey (1994)  A slow homotetrameric kinesin-related motor protein purified from Drosophila embryos. Journal of Biological Chemistry 269:22913-22916. PDF

 

Rashid, D.J.,  K.P. Wedaman and J.M. Scholey (1995)  Heterodimerization of the two motor subunits of the heterotrimeric kinesin, KRP 85/95.  Journal of Molecular Biology 252:157-162. PDF

 

Henson, J.H., D.G. Cole, M. Terasaki, D.J. Rashid and J.M. Scholey (1995) Immunolocalization of the hetrotrimeric kinesin-like protein, KRP 85/95 to the mitotic apparatus of sea urchin embryos.  Developmental Biology 171(1):182-194 PDF   

 

Kashina, A.S., R.J. Baskin, D.G. Cole, K.P. Wedaman, W.M. Saxon and J.M. Scholey (1996)  A bipolar kinesin.  Nature 379:270-272. PDF

 

Scholey, J.M. (1996)  Kinesin-II, A Membrane Traffic Motor in Axons, Axonemes and Spindles. J Cell Biol. 133:1-4 PDF

 

Kashina, A.S., J.M. Scholey, J.D. Leszyk and W.M. Saxton (1996b)  An essential bipolar mitotic motor.  Nature 384:225. PDF

 

Wedaman, K.P., D.W. Meyer, D.J. Rashid, D.G. Cole and J.M. Scholey (1996)  Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex.  Journal of Cell Biology 132(3):371-380. PDF

 

Bi, G-Q., R.L. Morris, G. Liao, J.M. Alderton, J.M. Scholey and R.A. Steinhardt (1997)  Kinesin- and myosin-driven steps of vesicle recruitment for CA2+-regulated exocytosis.  Journal of Cell Biology 138(5):999-1008. PDF

 

Morris, R.L. and J.M. Scholey (1997)  Heterotrimeric kinesin-II is required for the assembly of motile 9+2 ciliary axonemes on sea urchin embryos.  Journal of Cell Biology 138(5):1009-1022. PDF

 

Sharp, D. J., K.L. McDonald, H.M. Brown, H.J. Matthies, C. Walczak, R.D. Vale, T.J. Mitchison and J.M. Scholey (1999)  The bipolar kinesin, KLP61F, cross-links microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic spindles.  Journal of Cell Biology 144:125-138. PDF

 

Signor, D., K.P. Wedaman, L.S. Rose and J.M. Scholey (1999) Two heteromeric kinesin complexes in chemosensory neurons and sensory cilia of Caenorhabditis elegans. Molecular Biology of the Cell 10:345-360. PDF

 

Orozco, J., K.P. Wedaman, D. Signor, H. Brown, L.S. Rose and J.M. Scholey (1999) Movement of motor and cargo along cilia. Nature 398:674. PDF

 

Sharp, D.J., C. Yu, J. Sisson, W. Sullivan and J.M. Scholey (1999) Antagonistic microtubule sliding motors position mitotic centrosomes in Drosophila embryos.  Nature Cell Biology 1:50-54. PDF

 

Signor, D., K.P. Wedaman, J.T. Orozco, N.D. Dwyer, C. I. Bargmann, L.S. Rose, and J.M. Scholey (1999).  Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans.  Journal of Cell Biology. 147:519-530. PDF

 

Signor, D. and J.M. Scholey (2000) Microtubule-based transport in axons, dendrites and axonemes.  Essays in Biochemistry. 35:89-102.

 

Sharp, D.J., H. Brown, M. Kwon, G.C. Rogers, G. Holland and J.M. Scholey  (2000) Functional coordination of three mitotic motors in Drosophila embryos. Molecular Biology of the Cell. 11:241-253. PDF

 

Sharp, D.J., G.C. Rogers and J.M. Scholey (2000) Cytoplasmic dynein is required for poleward chromosome movement during mitosis in Drosophila embryos. Nature Cell Biology2: 922-930. PDF

 

Sharp, D.J., G.C. Rogers and J.M. Scholey. (2000) Microtubule-motors in mitosis. Nature407: 41-47. (Commissioned review article) PDF

 

Signor, D., Rose, L.S., and J.M. Scholey (2000) Analysis of the roles of kinesin and dynein motors in microtubule-based transport in the C.elegans nervous system.  Methods 22:317-325. PDF

 

Zhou, H.M., Brust-Mascher, I., and J.M. Scholey (2001) Direct visualization of the movement of the monomeric axonal transport motor, UNC-104, along  neuronal processes in living C. elegans. Journal of Neuroscience 21:3749-3755. PDF

 

Scholey, J.M., Rogers, G.C., and Sharp, D.J. (2001) Mitosis, microtubules, and  the matrix. Journal of Cell Biology 154: 261-266. PDF

 

Brust-Mascher, I., Scholey J.M. (2002) Microtubule flux and sliding in mitotic spindles of Drosophila embryos. Molecular Biology of the Cell. 13:3967-3975.  PDF

 

Cytrynbaum, E.N., Scholey, J.M., and A. Mogilner.  (2003). A force balance model of early spindle pole separation in Drosophila embryos. Biophysical Journal. 84: 757-769. PDF

 

Scholey, J.M., Brust-Mascher, I., Mogilner A. (2003) Cell division.  Nature. 422:   746-52. Review. PDF

 

Scholey, J.M. (2003) Intraflagellar Transport. Ann. Rev. Cell. Dev. Biol. 19: 423-443. PDF

 

Kwon, M.J., S. Morales-Mulia, I. Brust-Mascher, G.C. Rogers, D.J. Sharp, and J.M. Scholey (2004). The chromokinesin, KLP3A, drives spindle pole separation during prometaphase and anaphase, and facilitates chromatid motility. Molecular Biology of the Cell15(1):219-33. PDF

 

Rogers GC, Rogers SL, Schwimmer TA, Ems-McClung SC, Walczak CE, Vale RD, Scholey JM, Sharp DJ. (2004) Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase.  Nature.  427(6972):364-70. PDF

 

Lawrence, C.J., Dawe, R.K., Christie, K.R., Cleveland, D.W., Dawson, S.C., Endow, S.A., Goldstein, L.S.B., Goodson, H.V., Hirokawa, N., Howard, J., Malmbert, R.L., McIntosh, J.R., Miki, H., Mitchison, T.J., Okada, Y., Reddy, A.S.N. Saxton, W.M., Schliwa, M., Scholey, J.M., Vale, R.D., Walczak, C.E. and Wordeman, L. (2004) A Standardized Kinesin Nomenclature. Journal of Cell Biology. 167: 19-22.  PDF

 

Snow, J, Ou, G, Gunnarson, A, Walker, R, Zhou, HM, Brust-Mascher, I and Scholey JM (2004) Two Anterograde Intraflagellar Transport Motors Cooperate to Build Sensory Cilia on C. elegans Neurons. Nature Cell Biol. 6 (11):1109-1113 PDF

 

Brust-Mascher, I., Civelekoglu-Scholey, G., Kwon, M., Mogilner, A., and Scholey, J.M. (2004) Model for Anaphase B: Role of three mitotic motors in a switch from poleward flux to spindle elongation. Proc. Natl. Acad. Sci (USA). 101 (45) 15938-15943. PDF

 

Guangshuo Ou, Oliver E. Blacque, Joshua J. Snow, Michel R. Leroux & Jonathan M. Scholey. (2005) Functional coordination of intraflagellar transport motors. Nature. 2005 Jul 28;436(7050):583-7. PDF

 

Civelekoglu-Scholey, G., Sharp, DJ, Mogilner, A., and Scholey, J.M. (2006) Model of chromosome motility in Drosophila embryos: adaptation of a general mechanism for rapid mitosis.  Biophys. J. 90(11):3966-82. PDF

 

Pan, X., Ou G., Civelekoglu-Scholey, G.,Blaque O.E., Endres N.F., Tao, L, Mogilner, A., Leroux, M.R., Vale, R., and Scholey, J.M. (2006) Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. J. Cell Biol. 174(7):1035-45. PDF

 

Tao L, Mogilner A, Civelekoglu-Scholey G, Wollman R, Evans J, Stahlberg H, Scholey JM. (2006) A Homotetrameric Kinesin-5, KLP61F, Bundles Microtubules and Antagonizes Ncd in Motility Assays.
Curr. Biol. 16(23):2293-302. PDF

 

Cheerambathur DK, G Civelekoglu-Scholey, I Brust-Mascher, P Sommi, A Mogilner, and J.M. Scholey. (2007)  Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient. J Cell Biol. 177:995-1004 PDF

 

Civelekoglu-Scholey G and J.M. Scholey (2007)  Mitotic Motors: Kinesin-5 Takes a Brake. Curr. Biol. 17:R544-R547 PDF

 

Brust-Mascher, I and JM Scholey. (2007) Mitotic Spindle Dynamics in Drosophila. Int. Rev. of Cytology, 259: 139-172.

 

Wollman R, Civelekoglu-Scholey G, Scholey JM and A Mogilner (2008) Reverse engineering of force integration during mitosis in the Drosophila embryo. Molecular Systems Biology 4: 195. PDF

 

Cheerambathur DK, I Brust-Mascher, G Civelekoglu-Scholey, and J.M. Scholey. (2008) Dynamic partitioning of mitotic kinesin-5 cross-linkers between microtubule-bound and freely diffusing states. J Cell Biol. 182:429-426. PDF

 

van den Wildenberg, SMJL, L Tao, LC Kapitein, CF Schmidt, JM Scholey, and EJG Peterman. (2008)  The Homotetrameric Kinesin-5 KLP61F Preferentially Crosslinks Microtubules into Antiparallel Orientations. Curr. Biol. 18: 18601864. PDF

 

Brust-Mascher, I, P. Sommi, D.K. Cheerambathur, and J.M. Scholey (2009) Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.  Mol. Biol. Cell 20: 1749-1762. PDF

 

Brust-Mascher, I and JM Scholey. (2009) Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo. JoVE. 31. http://www.jove.com/index/details.stp?id=1382, doi: 10.3791/1382

 

Civelekoglu-Scholey G,  L Tao, I Brust-Mascher, R Wollman, and J.M. Scholey. (2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.  J Cell Biol. 188:49-68. PDF

 

Wang, H, I Brust-Mascher, D Cheerambathur and J.M. Scholey (2010) Coupling Between Microtubule Sliding, Plus-End Growth and Spindle Length Revealed by Kinesin-8 Depletion. Cytoskeleton 67:715-728. PDF

 

Goshima, G. and J.M. Scholey (2010) Control of Mitotic Spindle Length. Annual Rev. of Cell and Dev. Biol. 26: 21-57. doi: 10.1146/annurev-cellbio-100109-104006

  Hao, L., M. Thein, I. Brust-Mascher, G. Civelekoglu-Scholey, Y. Lu, S. Acar, B. Prevo, S. Shaham, and J.M. Scholey (2011) Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments. Nature Cell Biol. 13(7):790-8 doi: 10.1038/ncb2268 PDF
  Acar, S., D.B. Carlson, M.S. Budamagunta, V. Yarov-Yarovoy, J.J. Correia, M.R. Ninonuevo, W. Jia, L. Tao, J.A. Leary, J.C. Voss, J.E. Evans and J.M. Scholey (2013) The bipolar assembly domain of the mitotic motor kinesin-5. Nature Communications 4:1342 doi: 10.1038/ncomms2348 PDF
  Scholey, J.M.(2013) Kinesin-2: A family of heterotrimeric and homodimeric motors with diverse intracellular transport functions. Annual Review of Cell and Developmental Biology. 29:443–69.
  Wang, H., Brust-Mascher I, Civelekoglu-Scholey G and Scholey JM (2013) Patronin Mediates a switch from Kinesin-13-dependent Poleward Flux to Anaphase B Spindle Elongation. J. Cell Biology. 203, 35-46.
  Scholey J.E., Nithianantham S., Scholey J.M. and Al-Bassam J. (2014) Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers. eLife 3:e02217.
  Wang, H., Brust-Mascher I, and Scholey JM (2015) The microtubule cross-linker Feo, controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos. Mol. Biol. Cell. 26, 1456-52.
  Brust-Mascher I, Civelekoglu-Scholey G and Scholey JM (2015) Mechanism for Anaphase B: Evaluation of "Slide-and-Cluster" versus "Slide-and-Flux-or-Elongate" Models. Biophys. J. 108, 2007-18. PDF